NASC Stock Detail Page
NASC ID: N558315
Name: SALK_058315
ABRC stock number: SALK_058315
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. April 2002 TAIR gene annotation: K11I1.13 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g46540. Associated Polymorphisms: SALKseq_058315.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G46540.1 (at chr 5 pos 18878632 (W/18878580-18878632) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 32, R 33, C 32 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO389082. SALKseq_058315.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G30230.1 (at chr 1 pos 10639416 (W/10639339-10639416) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 32, R 33, C 32 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO379037. SALKseq_058315.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G04630.1=P355 and AT2G04650.1=UTR3 (at chr 2 pos 1621641 (C/1621641-1621701) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 32, R 33, C 32 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO382382. SALKseq_058315.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G42160.1 (at chr 2 pos 17575509 (W/17575456-17575509) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 32, R 33, C 32 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO385735.
Donation Date: 2002-04-30
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_058315
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_058315C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.