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The European Arabidopsis Stock Centre

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NASC ID: N559819

Name: SALK_059819

ABRC stock number: SALK_059819

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: F15N18.100 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g11510.

Associated Polymorphisms:

SALKseq_059819.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G11510.1 (at chr 5 pos 3680486 (W/3680437-3680486) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 51-75 bps onto the T-DNA. Pools P 34, R 34, C 36 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO359025.

SALKseq_059819.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G65810.2 and AT1G65820.1=P355 (at chr 1 pos 24480898 (C/24480898-24480954) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 34, R 34, C 36 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO348997.

SALKseq_059819.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G45775.1 (at chr 5 pos 18565474 (W/18565409-18565474) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 34, R 34, C 36 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO352352.

SALKseq_059819.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G65530.1 (at chr 5 pos 26191026 (W/26190969-26191026) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 34, R 34, C 36 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO355684.

Donation Date: 2002-04-30

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_059819

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N655554 (SALK_059819C).

Status Price (£)
Available £7.50

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.