NASC Stock Detail Page
NASC ID: N559967
Name: SALK_059967
ABRC stock number: SALK_059967
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. March 2002 TAIR gene annotation: T22E16.210 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g55550. Associated Polymorphisms: SALKseq_059967.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G55550.1 (at chr 3 pos 20602160 (W/20602100-20602160) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 35, R 33, C 31 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342305. SALKseq_059967.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G17723.1 and AT2G17730.1=P355 (at chr 2 pos 7702924 (C/7702924-7702987) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 35, R 33, C 31 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409210. SALKseq_059967.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G26400.1 (at chr 4 pos 13345480 (W/13345419-13345480) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 35, R 33, C 31 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO345660. SALKseq_059967.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G49142.1 and AT3G49150.1 (at chr 3 pos 18218025 (W/18217988-18218025) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 35, R 33, C 31 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412563. SALKseq_059967.201 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G13130.1 (at chr 5 pos 4170254 (W/4170213-4170254) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 39-68 bps onto the T-DNA. Pools P 35, R 33, C 31 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO349005.
Donation Date: 2002-03-21
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_059967
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666184 (SALK_059967C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.