NASC Stock Detail Page
NASC ID: N561267
Name: SALK_061267
ABRC stock number: SALK_061267
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. April 2002 TAIR gene annotation: F6N18.4 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g32640. Associated Polymorphisms: SALKseq_061267.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G32640.1 (at chr 1 pos 11800038 (C/11800038-11800100) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 31, R 33, C 38 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO405953. SALKseq_061267.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G23910.1 and AT1G23920.1=P355 (at chr 1 pos 8452154 (W/8452108-8452154) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 31, R 33, C 38 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409292. SALKseq_061267.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G13235.1 and AT4G13240.1 (at chr 4 pos 7679768 (W/7679718-7679768) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 51-77 bps onto the T-DNA. Pools P 31, R 33, C 38 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412644.
Donation Date: 2002-04-30
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_061267
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N656547 (SALK_061267C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.