NASC

NASC ID: N561308

Name: SALK_061308

ABRC stock number: SALK_061308

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: MDK4.15 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g54330.

Associated Polymorphisms:

SALKseq_061308.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G54330.1 (at chr 5 pos 22065334 (W/22065257-22065334) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 23, R 29, C 30 N 30. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO385908.

SALKseq_061308.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G56061=P355 and AT5G56070.1 (at chr 5 pos 22706229 (W/22706179-22706229) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 52-76 bps onto the T-DNA. Pools P 23, R 29, C 30 N 30. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO389255.

SALKseq_061308.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G35790.1 (at chr 4 pos 16957015 (W/16956938-16957015) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 23, R 29, C 30 N 30. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO382554.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_061308

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_061308C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT4G35790 AT5G56061 AT5G56070 At5g54330

EMBL accession:

ED591824

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.