NASC

NASC ID: N561383

Name: SALK_061383

ABRC stock number: SALK_061383

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: AP22.14 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At4g36400.

Associated Polymorphisms:

SALKseq_061383.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G36400.1 (at chr 4 pos 17200013 (W/17199951-17200013) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 36, R 32, C 37 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342392.

SALKseq_061383.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G53650.1=P355 and AT1G53660.1 (at chr 1 pos 20032287 (W/20032259-20032287) on the TAIR10). The sequences' 1-29 bps mapped on the genome, 43-78 bps onto the T-DNA. Pools P 36, R 32, C 37 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409298.

SALKseq_061383.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G57500.1 (at chr 3 pos 21281596 (W/21281543-21281596) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 56-77 bps onto the T-DNA. Pools P 36, R 32, C 37 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412650.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_061383

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_061383C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G53650 AT1G53660 AT3G57500 At4g36400

EMBL accession:

ED591871

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.