NASC Stock Detail Page
NASC ID: N561391
Name: SALK_061391
ABRC stock number: SALK_061391
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. April 2002 TAIR gene annotation: F2H17.16 R300-5' At2g36240 F2H17.15 L300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At2g36230. Associated Polymorphisms: SALKseq_061391.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G36230.1 (at chr 2 pos 15195543 (W/15195484-15195543) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 35, R 40, C 40 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362467. SALKseq_061391.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G23496.1=P355 and AT4G23500.1=P355 (at chr 4 pos 12262077 (W/12261999-12262077) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 35, R 40, C 40 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO365817. SALKseq_061391.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G23590.1=UTR3 and AT4G23600.1=P355 (at chr 4 pos 12310007 (C/12310007-12310049) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 35, R 40, C 40 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369160. SALKseq_061391.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G09230.1 (at chr 5 pos 2873520 (W/2873478-2873520) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 35, R 40, C 40 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO372517. SALKseq_061391.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G09230.1 (at chr 5 pos 2873524 (C/2873524-2873566) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 35, R 40, C 40 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO375871.
Donation Date: 2002-04-30
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_061391
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666212 (SALK_061391C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.