NASC Stock Detail Page
NASC ID: N562547
Name: SALK_062547
ABRC stock number: SALK_062547
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. April 2002 TAIR gene annotation: F11F12.17 L300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g50480. Associated Polymorphisms: SALKseq_062547.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G50480.1 (at chr 1 pos 18701921 (W/18701843-18701921) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 38, R 32, C 31 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409370. SALKseq_062547.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G44191.1 and AT1G44224.1=UTR3 (at chr 1 pos 16818228 (C/16818228-16818255) on the TAIR10). The sequences' 1-28 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 38, R 32, C 31 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO406031. SALKseq_062547.101 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G28500.1=UTR3 and AT5G28510.1 (at chr 5 pos 10479891 (W/10479813-10479891) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 38, R 32, C 31 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412722.
Donation Date: 2002-04-30
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_062547
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666225 (SALK_062547C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.