NASC

NASC ID: N563050

Name: SALK_063050

ABRC stock number: SALK_063050

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: T23O15.19 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At2g04340.

Associated Polymorphisms:

SALKseq_063050.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G04340.1 (at chr 2 pos 1515324 (W/1515270-1515324) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 37, R 31, C 40 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO365912.
SALKseq_063050.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G18340.1 (at chr 1 pos 6313402 (W/6313357-6313402) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 43-70 bps onto the T-DNA. Pools P 37, R 31, C 40 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362564.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_063050

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_063050C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G18340 At2g04340

EMBL accession:

ED593687

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.