NASC Stock Detail Page
NASC ID: N563374
Name: SALK_063374
ABRC stock number: SALK_063374
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. April 2002 TAIR gene annotation: MPE11.26 R300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g26085. Associated Polymorphisms: SALKseq_063374.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G13510.1=UTR3 and AT3G13520.1=P355 (at chr 3 pos 4406537 (C/4406537-4406586) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 49-73 bps onto the T-DNA. Pools P 38, R 39, C 31 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO389375. SALKseq_063374.001 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G26090.1 (at chr 3 pos 9532532 (W/9532474-9532532) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 38, R 39, C 31 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO392715. SALKseq_063374.1 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT2G15230.1 (at chr 2 pos 6612648 (W/6612599-6612648) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 51-78 bps onto the T-DNA. Pools P 38, R 39, C 31 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO386028.
Donation Date: 2002-04-30
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_063374
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666242 (SALK_063374C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.