NASC

NASC ID: N566772

Name: SALK_066772

ABRC stock number: SALK_066772

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_066772.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G07915.1 (at chr 4 pos 4718341 (W/4718258-4718341) on the TAIR10). The sequences' 1-84 bps mapped on the genome, 74-95 bps onto the T-DNA. Pools P 43, R 49, C 42 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412979.

SALKseq_066772.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G02074.1 and AT1G02080.2=P355 (at chr 1 pos 372750 (W/372705-372750) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 72-95 bps onto the T-DNA. Pools P 43, R 49, C 42 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO402957.

SALKseq_066772.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G17970.1 (at chr 1 pos 6184699 (C/6184699-6184765) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 43, R 49, C 42 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO406285.

SALKseq_066772.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT2G39870.1 (at chr 2 pos 16643616 (C/16643616-16643691) on the TAIR10). The sequences' 1-76 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 43, R 49, C 42 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409624.

Donation Date: 2002-05-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_066772

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N655587 (SALK_066772C).

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G02074 AT1G02080 AT1G17970 AT2G39870 At4g07915

EMBL accession:

ED595873

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.