NASC Stock Detail Page
NASC ID: N567444
Name: SALK_067444
ABRC stock number: SALK_067444
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. June 2002 TAIR gene annotation - F17M19.14 Exon. At1g71990. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_067444.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G71990.1 (at chr 1 pos 27100880 (W/27100827-27100880) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 45, R 48, C 48 N 46. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409660. SALKseq_067444.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G64140.1 (at chr 1 pos 23807085 (W/23807034-23807085) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 45, R 48, C 48 N 46. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO406322. SALKseq_067444.101 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G34720.1 (at chr 2 pos 14650588 (C/14650588-14650628) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 45, R 48, C 48 N 46. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO413016.
Donation Date: 2002-01-06
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_067444
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666312 (SALK_067444C).
Status | Price (£) |
---|---|
Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
---|---|---|---|
|
|
|
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.