NASC Stock Detail Page
NASC ID: N584053
Name: SALK_084053
ABRC stock number: SALK_084053
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_084053.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G11580.1=UTR3 and AT1G11590.1=P355 (at chr 1 pos 3892151 (C/3892151-3892228) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 71, R 80, C 77 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO417950. SALKseq_084053.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G12630.1 and AT1G12640.1=UTR3 (at chr 1 pos 4301710 (W/4301660-4301710) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 71, R 80, C 77 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO421266. SALKseq_084053.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G53670.1 (at chr 5 pos 21797748 (W/21797703-21797748) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 42-78 bps onto the T-DNA. Pools: P 71, R 80, C 77 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424583. SALKseq_084053.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G57830.1=P355 and AT5G57840.1=UTR3 (at chr 5 pos 23431398 (W/23431307-23431398) on the TAIR10). The sequences' 1-92 bps mapped on the genome. Pools P 71, R 80, C 77 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO427898. SALKseq_084053.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G58840.1=UTR3 and AT5G58850.1 (at chr 5 pos 23763232 (C/23763232-23763309) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 71, R 80, C 77 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431214.
Donation Date: 2003-01-30
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_084053
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666577 (SALK_084053C).
Status | Price (£) |
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Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.