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NASC ID: N584171

Name: SALK_084171

ABRC stock number: SALK_084171

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

December 2002 TAIR gene annotation - At1g64250 F22C12.32 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_084171.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G64250.1 (at chr 1 pos 23842523 (W/23842480-23842523) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 49-77 bps onto the T-DNA. Pools P 70, R 70, C 61 N 65. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424608.

SALKseq_084171.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G51210.1 (at chr 3 pos 19021094 (W/19021017-19021094) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools: P 70, R 70, C 61 N 65. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO427923.

SALKseq_084171.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT3G26210.1 (at chr 3 pos 9593136 (W/9593076-9593136) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 70, R 70, C 61 N 65. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431239.

Donation Date: 2003-01-30

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_084171

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664727 (SALK_084171C).

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.