NASC

NASC ID: N584317

Name: SALK_084317

ABRC stock number: SALK_084317

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At4g32470. F8B4.170 R300-5' Gly-00004 Gly-00004 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_084317.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G32470.1 (at chr 4 pos 15671201 (W/15671165-15671201) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 52-78 bps onto the T-DNA. Pools P 73, R 71, C 74 N 71. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434577.
SALKseq_084317.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G32010.1=UTR3 and AT4G32020.1=P355 (at chr 4 pos 15486842 (W/15486781-15486842) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 60-77 bps onto the T-DNA. Pools P 73, R 71, C 74 N 71. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431271.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_084317

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N655676 (SALK_084317C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT4G32010 AT4G32020 At4g32470

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.