NASC

NASC ID: N584410

Name: SALK_084410

ABRC stock number: SALK_084410

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_084410.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G07334.1 (at chr 4 pos 4138690 (W/4138613-4138690) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 73, R 71, C 77 N 80. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO418028.

SALKseq_084410.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G50930.1 (at chr 3 pos 18929990 (W/18929941-18929990) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 73, R 71, C 77 N 80. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO434597.

SALKseq_084410.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G07870.1 (at chr 3 pos 2510765 (W/2510723-2510765) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 73, R 71, C 77 N 80. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO437901.

SALKseq_084410.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G17140.1 (at chr 5 pos 5637939 (W/5637877-5637939) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 73, R 71, C 77 N 80. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO421344.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_084410

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_084410C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G07870 AT3G50930 At4g07334 AT5G17140

EMBL accession:

BH855885

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.