NASC Stock Detail Page
NASC ID: N585291
Name: SALK_085291
ABRC stock number: SALK_085291
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_085291.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G44110.1=P355 and AT1G44125 (at chr 1 pos 16778089 (W/16778033-16778089) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 73, R 72, C 76 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO421511. SALKseq_085291.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G69020.1 (at chr 1 pos 25946591 (W/25946537-25946591) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 54-78 bps onto the T-DNA. Pools P 73, R 72, C 76 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424828. SALKseq_085291.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G27600.2=P355 and AT1G27610.1 (at chr 1 pos 9606673 (C/9606673-9606728) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 73, R 72, C 76 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428143. SALKseq_085291.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G29140.1 (at chr 2 pos 12535315 (W/12535258-12535315) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 49-78 bps onto the T-DNA. Pools P 73, R 72, C 76 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431458. SALKseq_085291.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G07610.2 (at chr 3 pos 2425998 (W/2425941-2425998) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 73, R 72, C 76 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434765.
Donation Date: 2003-01-30
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_085291
Part of : SALK lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.