NASC Stock Detail Page
NASC ID: N585932
Name: SALK_085932
ABRC stock number: SALK_085932
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. July 2002 TAIR gene annotation - At3g09570. F11F8.41 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_085932.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G29050.1=P355 and AT1G29060.1=P355 (at chr 1 pos 10145959 (W/10145894-10145959) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 75, R 75, C 75 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438183. SALKseq_085932.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G21260=P355 and AT1G21270.1=P355 (at chr 1 pos 7443710 (C/7443710-7443787) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 75, R 75, C 75 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO418310. SALKseq_085932.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G11964.1 (at chr 3 pos 3806703 (W/3806669-3806703) on the TAIR10). The sequences' 1-35 bps mapped on the genome, 48-78 bps onto the T-DNA. Pools P 75, R 75, C 75 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO421626. SALKseq_085932.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G03460.1 (at chr 5 pos 865584 (W/865528-865584) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 51-74 bps onto the T-DNA. Pools P 75, R 75, C 75 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424943.
Donation Date: 2002-01-07
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_085932
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666611 (SALK_085932C).
Status | Price (£) |
---|---|
Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
---|---|---|---|
|
|
|
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.