NASC Stock Detail Page
NASC ID: N587874
Name: SALK_087874
ABRC stock number: SALK_087874
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. August 2002 TAIR gene annotation - F1O11.11 R300-5', At2g36485. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_087874.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G36485.1 (at chr 2 pos 15307826 (C/15307826-15307876) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 77, R 76, C 79 N 73. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435246. SALKseq_087874.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G36960.3=UTR3 and AT2G36970.1=P355 (at chr 2 pos 15528320 (C/15528320-15528369) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 77, R 76, C 79 N 73. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438550. SALKseq_087874.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G13263.1=UTR3 and AT4G13260.1=UTR3 (at chr 4 pos 7717713 (C/7717713-7717774) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 77, R 76, C 79 N 73. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO418677. SALKseq_087874.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G11050.1=UTR3 and AT5G11060.1=P355 (at chr 5 pos 3507068 (W/3507004-3507068) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 77, R 76, C 79 N 73. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO421993. SALKseq_087874.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G22930.1 and AT5G22940.1=P355 (at chr 5 pos 7673099 (W/7673041-7673099) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 77, R 76, C 79 N 73. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO425310.
Donation Date: 2002-01-09
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_087874
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_087874C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.