NASC

NASC ID: N589912

Name: SALK_089912

ABRC stock number: SALK_089912

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

August 2002 TAIR gene annotation - T10D10.2 Exon, At1g72510. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_089912.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G72510.1 (at chr 1 pos 27304350 (C/27304350-27304424) on the TAIR10). The sequences' 1-75 bps mapped on the genome, 78-100 bps onto the T-DNA. Pools P 76, R 79, C 72 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO425687.

SALKseq_089912.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G75000.1 and AT1G75010.1=UTR3 (at chr 1 pos 28164656 (C/28164656-28164733) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 76, R 79, C 72 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429000.

SALKseq_089912.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G75500.1 (at chr 1 pos 28340385 (W/28340337-28340385) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 76, R 79, C 72 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432317.

SALKseq_089912.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G28340.1 (at chr 3 pos 10590724 (C/10590724-10590774) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 76, R 79, C 72 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435623.

Donation Date: 2002-01-09

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_089912

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666693 (SALK_089912C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G75000 AT1G75010 AT1G75500 AT3G28340 At1g72510

EMBL accession:

ED598462

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.