NASC Stock Detail Page
NASC ID: N590314
Name: SALK_090314
ABRC stock number: SALK_090314
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. August 2002 TAIR gene annotation - F21M11.30 Exon, At1g03790. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_090314.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G57590.1 (at chr 1 pos 21330111 (W/21330036-21330111) on the TAIR10). The sequences' 1-76 bps mapped on the genome, 75-95 bps onto the T-DNA. Pools P 78, R 80, C 73 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO422431. SALKseq_090314.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G23710.1=UTR3 and AT3G23715.1 (at chr 3 pos 8536580 (W/8536516-8536580) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 64-88 bps onto the T-DNA. Pools P 78, R 80, C 73 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO425748. SALKseq_090314.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G23730.1 (at chr 4 pos 12362686 (W/12362617-12362686) on the TAIR10). The sequences' 1-70 bps mapped on the genome, 74-95 bps onto the T-DNA. Pools P 78, R 80, C 73 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429061. SALKseq_090314.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G50330.1 (at chr 5 pos 20487106 (W/20487029-20487106) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 78, R 80, C 73 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432377.
Donation Date: 2002-01-09
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_090314
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663283 (SALK_090314C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.