NASC

NASC ID: N591614

Name: SALK_091614

ABRC stock number: SALK_091614

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.September 2002 TAIR gene annotation - F21P24.9 Intron At2g23030. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms:

SALKseq_091614.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G23030.1 (at chr 2 pos 9803973 (W/9803896-9803973) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 76, R 78, C 75 N 74. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO425942.
SALKseq_091614.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G48790.1=P355 and AT1G48800.1 (at chr 1 pos 18049082 (W/18049017-18049082) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 76, R 78, C 75 N 74. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO422625.

SALKseq_091614.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G23030.1 (at chr 2 pos 9803973 (W/9803896-9803973) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 76, R 78, C 75 N 74. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO425942.
SALKseq_091614.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G48790.1=P355 and AT1G48800.1 (at chr 1 pos 18049082 (W/18049017-18049082) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 76, R 78, C 75 N 74. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO422625.

Donation Date: 2002-01-10

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_091614

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653361 (SALK_091614C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G48790 AT1G48800 At2g23030

EMBL accession:

BZ290839

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.