NASC

NASC ID: N592037

Name: SALK_092037

ABRC stock number: SALK_092037

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_092037.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G25600.1 and AT5G25610.1=UTR3 (at chr 5 pos 8914060 (W/8913999-8914060) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 76, R 75, C 72 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO419363.

SALKseq_092037.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G32225.1 (at chr 1 pos 11610600 (C/11610600-11610639) on the TAIR10). The sequences' 1-40 bps mapped on the genome, 45-66 bps onto the T-DNA. Pools P 76, R 75, C 72 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435931.

SALKseq_092037.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G39010.1 (at chr 4 pos 18178169 (C/18178169-18178198) on the TAIR10). The sequences' 1-30 bps mapped on the genome, 33-57 bps onto the T-DNA. Pools P 76, R 75, C 72 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO439234.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_092037

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666738 (SALK_092037C).

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G32225 AT4G39010 At5g25600 AT5G25610

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.