NASC

NASC ID: N592046

Name: SALK_092046

ABRC stock number: SALK_092046

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_092046.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G51750 and AT1G51760.1=P355 (at chr 1 pos 19197935 (W/19197901-19197935) on the TAIR10). The sequences' 1-34 bps mapped on the genome, 38-62 bps onto the T-DNA. Pools P 76, R 75, C 71 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435932.

SALKseq_092046.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G38280.1 (at chr 5 pos 15296212 (W/15296160-15296212) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 76, R 75, C 71 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO439235.

SALKseq_092046.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G40981.1 and AT5G40990.1 (at chr 5 pos 16418694 (W/16418638-16418694) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 76, R 75, C 71 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO419364.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_092046

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N655727 (SALK_092046C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G51750 AT1G51760 At5g04280 AT5G38280 AT5G40981 AT5G40990

EMBL accession:

CC796016

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.