NASC Stock Detail Page
NASC ID: N606339
Name: SALK_106339
ABRC stock number: SALK_106339
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_106339.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G04240.1 (chr 3 pos 1120836 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KG788222. SALKseq_106339.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G30150.1 and AT2G30160.1-P355 (chr 2 pos 12876332 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KG788219. SALKseq_106339.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT2G41540.1 (chr 2 pos 17326330 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KG788220. SALKseq_106339.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G54380.1 (chr 3 pos 20134405 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KG788223. SALKseq_106339.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G58790.1 (chr 3 pos 21746565 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KG788221.
Donation Date: 2003-01-31
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_106339
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N658011 (SALK_106339C).
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.