NASC Stock Detail Page
NASC ID: N619862
Name: SALK_119862
ABRC stock number: SALK_119862
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_119862.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G28155.1 and AT3G28157.1=P355 (at chr 3 pos 10486360 (C/10486360-10486413) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 51, R 56, C 54 N 59. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO353268. SALKseq_119862.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G01335.1 (at chr 5 pos 137122 (C/137122-137181) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 51, R 56, C 54 N 59. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO356589. SALKseq_119862.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G39380.1 and AT5G39390.1 (at chr 5 pos 15763584 (W/15763526-15763584) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 51, R 56, C 54 N 59. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO359935. SALKseq_119862.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G01490.1 (at chr 5 pos 196124 (C/196124-196157) on the TAIR10). The sequences' 1-34 bps mapped on the genome, 29-79 bps onto the T-DNA. Pools P 51, R 56, C 54 N 59. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO363287. SALKseq_119862.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G13030.1 (at chr 5 pos 4135905 (W/4135857-4135905) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 50-79 bps onto the T-DNA. Pools P 51, R 56, C 54 N 59. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO366634.
Donation Date: 2002-11-01
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_119862
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N667156 (SALK_119862C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
|---|---|---|---|
|
|
|
|
Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.