NASC

NASC ID: N624736

Name: SALK_124736

ABRC stock number: SALK_124736

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At3g20710 F3H11.1 1 Exon F3H11.1 1 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_124736.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G20710.1 (at chr 3 pos 7238595 (W/7238517-7238595) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 54, R 59, C 58 N 57. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO353454.

SALKseq_124736.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G34950.1=P355 and AT4G34960.1=P355 (at chr 4 pos 16645414 (W/16645336-16645414) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 54, R 59, C 58 N 57. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO356780.

SALKseq_124736.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G36330=P355 and AT1G36340.1=UTR3 (at chr 1 pos 13682068 (W/13682016-13682068) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 54, R 59, C 58 N 57. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO346756.

SALKseq_124736.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G19710.1 (at chr 1 pos 6814613 (W/6814550-6814613) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 54, R 59, C 58 N 57. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO350105.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_124736

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664871 (SALK_124736C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G19710 AT1G36330 AT1G36340 AT4G34950 AT4G34960 At3g20710

EMBL accession:

BZ764462

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.