NASC

NASC ID: N627179

Name: SALK_127179

ABRC stock number: SALK_127179

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At3g22230 MKA23.22 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_127179.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G22230.1 (at chr 3 pos 7844582 (W/7844517-7844582) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 71-95 bps onto the T-DNA. Pools P 57, R 53, C 51 N 55. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO350235.

SALKseq_127179.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G04080.1 (at chr 1 pos 1053531 (W/1053482-1053531) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 51-77 bps onto the T-DNA. Pools P 57, R 53, C 51 N 55. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO410441.

SALKseq_127179.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G09310.1 (at chr 1 pos 3008677 (W/3008636-3008677) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 72-91 bps onto the T-DNA. Pools P 57, R 53, C 51 N 55. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO413796.

SALKseq_127179.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G22790.1 (at chr 1 pos 8070636 (C/8070636-8070706) on the TAIR10). The sequences' 1-71 bps mapped on the genome, 76-95 bps onto the T-DNA. Pools P 57, R 53, C 51 N 55. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO343544.

SALKseq_127179.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G14260.1=P355 and AT3G14270.1=P355 (at chr 3 pos 4751699 (W/4751636-4751699) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 57, R 53, C 51 N 55. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO346890.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_127179

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_127179C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G04080 AT1G09310 AT1G22790 AT3G14260 AT3G14270 At3g22230

EMBL accession:

BZ355670

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.