NASC

NASC ID: N628619

Name: SALK_128619

ABRC stock number: SALK_128619

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At3g51090 F24M12.130 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_128619.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G51090.1 (at chr 3 pos 18979104 (C/18979104-18979165) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 58, R 52, C 53 N 54. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO397153.

SALKseq_128619.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G36310.1 (at chr 1 pos 13670661 (W/13670583-13670661) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 58, R 52, C 53 N 54. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390462.

SALKseq_128619.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G40981.1 and AT5G40990.1 (at chr 5 pos 16416184 (W/16416128-16416184) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 58, R 52, C 53 N 54. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400497.

SALKseq_128619.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G27650.2=UTR3 and AT1G27660.1=P355 (at chr 1 pos 9620156 (C/9620156-9620220) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 50-79 bps onto the T-DNA. Pools P 58, R 52, C 53 N 54. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO393816.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_128619

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663955 (SALK_128619C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G27650 AT1G27660 AT1G36310 AT5G40981 AT5G40990 At3g51090

EMBL accession:

BZ292945

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.