NASC

NASC ID: N631657

Name: SALK_131657

ABRC stock number: SALK_131657

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_131657.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G35230.1 (at chr 1 pos 12916824 (W/12916750-12916824) on the TAIR10). The sequences' 1-75 bps mapped on the genome, 72-95 bps onto the T-DNA. Pools P 60, R 60, C 56 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400638.

SALKseq_131657.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G01830.3=P355 and AT2G01840.1 (at chr 2 pos 373023 (W/372960-373023) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 62-92 bps onto the T-DNA. Pools P 60, R 60, C 56 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO403979.

SALKseq_131657.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G20470.1 (around chr 4 pos 11032722 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO416409.

SALKseq_131657.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G20730.1 (at chr 4 pos 11120686 (C/11120686-11120764) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 60, R 60, C 56 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO410654.

SALKseq_131657.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G37420.1 (at chr 4 pos 17594287 (W/17594250-17594287) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 37-68 bps onto the T-DNA. Pools P 60, R 60, C 56 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO414004.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_131657

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_131657C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G35230 AT2G01830 AT2G01840 AT4G20470 AT4G20730 AT4G37420 At1g32770

EMBL accession:

BZ765540

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.