NASC

NASC ID: N632037

Name: SALK_132037

ABRC stock number: SALK_132037

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At1g58032 T15M6.2 5 Intron T15M6.2 5 Intron. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_132037.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G58030.1 (at chr 1 pos 21465410 (C/21465410-21465482) on the TAIR10). The sequences' 1-73 bps mapped on the genome, 79-100 bps onto the T-DNA. Pools P 25, R 22, C 20 N 21. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO340764.

SALKseq_132037.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G49450.1 (at chr 1 pos 18306609 (W/18306545-18306609) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 82-100 bps onto the T-DNA. Pools P 25, R 22, C 20 N 21. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO340054.

SALKseq_132037.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G69410.1 (at chr 1 pos 26088907 (W/26088826-26088907) on the TAIR10). The sequences' 1-82 bps mapped on the genome, 76-100 bps onto the T-DNA. Pools P 25, R 22, C 20 N 21. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO340394.

SALKseq_132037.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G18950.1-UTR3 and AT2G18960.1-P355 (at chr 2 pos 8210795 (W/8210727-8210795) on the TAIR10). The sequences' 1-69 bps mapped on the genome, 76-100 bps onto the T-DNA. Pools P 25, R 22, C 20 N 21. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO340055.

SALKseq_132037.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G23850.1 (at chr 5 pos 8039900 (W/8039834-8039900) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 79-100 bps onto the T-DNA. Pools P 25, R 22, C 20 N 21. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO340395.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_132037

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_132037C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G49450 At1g58030 AT1G58030 AT1G69410 AT2G18950 AT2G18960 AT5G23850 At1g58032

EMBL accession:

BZ358173

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.