NASC Stock Detail Page
NASC ID: N632434
Name: SALK_132434
ABRC stock number: SALK_132434
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. February 2003 TAIR gene annotation - At3g11800 F26K24. 9 L300-5' F26K24. 9 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_132434.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G11800.1 (at chr 3 pos 3726100 (W/3726064-3726100) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 41-79 bps onto the T-DNA. Pools P 15, R 12, C 19 N 11. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO347127. SALKseq_132434.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G22080.1 (at chr 4 pos 11702961 (W/11702883-11702961) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 15, R 12, C 19 N 11. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO350474. SALKseq_132434.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G53330.1 and AT1G53340.1 (at chr 1 pos 19898360 (W/19898308-19898360) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 52-79 bps onto the T-DNA. Pools P 15, R 12, C 19 N 11. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO414030. SALKseq_132434.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G28680.1=P355 and AT3G28685=P355 (at chr 3 pos 10750815 (W/10750737-10750815) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 15, R 12, C 19 N 11. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO343783.
Donation Date: 2003-03-11
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_132434
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664029 (SALK_132434C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.