NASC Stock Detail Page
NASC ID: N632562
Name: SALK_132562
ABRC stock number: SALK_132562
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. February 2003 TAIR gene annotation - At3g19580 MMB12.2 7 Exon MMB12.2 7 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_132562.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G19580.1 (at chr 3 pos 6803322 (W/6803256-6803322) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 65-83 bps onto the T-DNA. Pools P 25, R 25, C 20 N 23. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO340460. SALKseq_132562.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G54210.1 (at chr 3 pos 20068507 (W/20068427-20068507) on the TAIR10). The sequences' 1-81 bps mapped on the genome, 79-100 bps onto the T-DNA. Pools P 25, R 25, C 20 N 23. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO340459. SALKseq_132562.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G56680.1 (at chr 3 pos 20991262 (W/20991234-20991262) on the TAIR10). The sequences' 1-29 bps mapped on the genome, 33-83 bps onto the T-DNA. Pools P 25, R 25, C 20 N 23. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO340118.
Donation Date: 2003-03-11
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_132562
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N667366 (SALK_132562C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.