NASC Stock Detail Page
NASC ID: N633048
Name: SALK_133048
ABRC stock number: SALK_133048
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. February 2003 TAIR gene annotation - At5g64950 MXK3.18 E xon MXK3.18 E xon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_133048.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G64950.1 (at chr 5 pos 25954040 (W/25953980-25954040) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 38, R 31, C 39 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO367223. SALKseq_133048.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G37537 and AT1G37603.1=P355 (around chr 1 pos 14242301 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO416434. SALKseq_133048.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G40101.1 (around chr 1 pos 15069905 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO416435. SALKseq_133048.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G04410.1 (at chr 2 pos 1534191 (W/1534133-1534191) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 57-77 bps onto the T-DNA. Pools P 38, R 31, C 39 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO360524. SALKseq_133048.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G33058.1 (at chr 3 pos 13603136 (C/13603136-13603181) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 50-76 bps onto the T-DNA. Pools P 38, R 31, C 39 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO363871.
Donation Date: 2003-03-11
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_133048
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N655960 (SALK_133048C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.