NASC Stock Detail Page
NASC ID: N633531
Name: SALK_133531
ABRC stock number: SALK_133531
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. February 2003 TAIR gene annotation - At1g13980 F7A19.7 R 300-3' At1g13990 F7A19.8 L300-5' F7A19.7 R 300-3' At1g13990 F7A19.8 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_133531.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G13990.1 (at chr 1 pos 4794592 (W/4794520-4794592) on the TAIR10). The sequences' 1-73 bps mapped on the genome, 76-95 bps onto the T-DNA. Pools P 43, R 48, C 46 N 44. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373945.
SALKseq_133531.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G34210.1 (at chr 1 pos 12458592 (C/12458592-12458659) on the TAIR10). The sequences' 1-68 bps mapped on the genome, 69-95 bps onto the T-DNA. Pools P 43, R 48, C 46 N 44. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO370595.
Donation Date: 2003-03-11
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_133531
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_133531C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.