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NASC ID: N637224

Name: SALK_137224

ABRC stock number: SALK_137224

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At3g12370 T2E22.3 7 R300-5' At3g12380 T2E22.30 L300-3' T2E22.3 7 R300-5' At3g12380 T2E22.30 L300-3'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_137224.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G12370.1 (at chr 3 pos 3938146 (W/3938061-3938146) on the TAIR10). The sequences' 1-86 bps mapped on the genome, 82-100 bps onto the T-DNA. Pools P 26, R 26, C 28 N 29. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO340662.
SALKseq_137224.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G54865-P355 and AT5G54870.1-P355 (at chr 5 pos 22288281 (W/22288206-22288281) on the TAIR10). The sequences' 1-74 bps mapped on the genome, 65-83 bps onto the T-DNA. Pools P 26, R 26, C 28 N 29. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO340321.

Donation Date: 2003-03-11

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_137224

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_137224C.

Status Price (£)
Available to order at NASC £7.50

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.