NASC Stock Detail Page
NASC ID: N637974
Name: SALK_137974
ABRC stock number: SALK_137974
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_137974.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G31010.1 (at chr 2 pos 13197101 (W/13197024-13197101) on the TAIR10). The sequences' 3-80 bps mapped on the genome, 74-95 bps onto the T-DNA. Pools P 73, R 78, C 78 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO394244. SALKseq_137974.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G47460 and AT1G47465.1 (at chr 1 pos 17405140 (C/17405140-17405219) on the TAIR10). The sequences' 1-80 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 73, R 78, C 78 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO387548. SALKseq_137974.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G25580.1 (at chr 2 pos 10889163 (W/10889091-10889163) on the TAIR10). The sequences' 1-73 bps mapped on the genome, 72-95 bps onto the T-DNA. Pools P 73, R 78, C 78 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390890. SALKseq_137974.201 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G09960.4=P355 and AT4G09965.1 (at chr 4 pos 6242752 (W/6242695-6242752) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 70-95 bps onto the T-DNA. Pools P 73, R 78, C 78 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO397584. SALKseq_137974.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G53230.1 and AT5G53240.1=P355 (at chr 5 pos 21600536 (W/21600483-21600536) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 69-95 bps onto the T-DNA. Pools P 73, R 78, C 78 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400929.
Donation Date: 2003-03-11
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_137974
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_137974C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.