NASC

NASC ID: N638685

Name: SALK_138685

ABRC stock number: SALK_138685

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At1g80740 F23A5.9 I ntron F23A5.9 I ntron. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_138685.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G80740.1 (at chr 1 pos 30346148 (W/30346070-30346148) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 36, R 36, C 32 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO380894.

SALKseq_138685.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G14930.1=P355 and AT1G14940.1=UTR3 (at chr 1 pos 5154411 (W/5154361-5154411) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 51-78 bps onto the T-DNA. Pools P 36, R 36, C 32 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO384236.

SALKseq_138685.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G27210.1 (at chr 2 pos 11636608 (W/11636552-11636608) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 36, R 36, C 32 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO387586.

SALKseq_138685.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G02150.1 (at chr 3 pos 391172 (W/391133-391172) on the TAIR10). The sequences' 1-40 bps mapped on the genome, 41-69 bps onto the T-DNA. Pools P 36, R 36, C 32 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390927.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_138685

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_138685C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G14930 AT1G14940 AT2G27210 AT3G02150 At1g80740

EMBL accession:

BZ767324

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.