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The European Arabidopsis Stock Centre

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NASC ID: N832190

Name: SAIL_724_E08

ABRC stock number: CS832190

Description: T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pDAP101, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection.

Associated Polymorphisms:

SAILseq_724_E08.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G47450.1 (at chr 5 pos 19248514 (W/19248440-19248514) on the TAIR10). The sequences' 1-75 bps mapped on the genome, 78-100 bps onto the T-DNA. Pools P 33, R 34, C 34 N 34. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO335451.

SAIL_724_E08

Donation Date: 2004-06-01

Donated by:  Syngenta AG Alberto Martinez

Donor Number: SAIL_724_E08

Part of : SAIL lines from Syngenta

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SAIL_724_E08.

Status Price (£)
Available to order at NASC £7.50

Germplasm Info

Background: Col-0( N60000)

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

References

McElver, J. et al. 2001. Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana. Genetics 159(4):1751-63.PubMed ID: 11779812.