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NASC ID: N838676

Name: SAIL_861_H12

ABRC stock number: CS838676

Description: T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pDAP101, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection.

Associated Polymorphisms:

SAILseq_861_H12.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G45500.2-P355 and AT5G45510.1-P355 (at chr 5 pos 18435869 (C/18435869-18435942) on the TAIR10). The sequences' 1-74 bps mapped on the genome, 75-99 bps onto the T-DNA. Pools P 38, R 31, C 33 N 32. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO327585.

SAIL_861_H12

Donation Date: 2004-06-04

Donated by:  Syngenta AG Alberto Martinez

Donor Number: SAIL_861_H12

Part of : SAIL lines from Syngenta

Stock type: individual line

Material type: seed

Status Price (£)
Available to order at NASC £10.00

Germplasm Info

Background: Col-0( N60000)

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

References

McElver, J. et al. 2001. Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana. Genetics 159(4):1751-63.PubMed ID: 11779812.