NASC Stock Detail Page
NASC ID: N869848
Name: SALK_131324.2
ABRC stock number: CS869848
Description: confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
Donation Date: 2015-06-19
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALKseq_131324.2
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Donors: Joseph R. Ecker. Clone Name: pROK2. 2015-02-18 GenBank annotation - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G55040.2-P355 and AT5G55050.1-P355 (at chr 5 pos 22332775 (W/22332724-22332775) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 45-83 bps onto the T-DNA. Pools: P 25, R 20, C 29 N 26. The insertion site is at the 3' end of the sequence mapping. GenBank Acc: KO340053
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Background: Col-0(N60000),SALK_131324(N631324)
Growth requirement: none