NASC

NASC ID: N849118

Name: WiscDsLox4G07

ABRC stock number: CS849118

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox4G07 .

Wiscseq_DsLox4G07.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G44050.1 (chr 3 pos 15821510 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG809182.

Wiscseq_DsLox4G07.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G10070.1 (at chr 5 pos 3150192 (C/3150192-3150265) on the TAIR10). The sequences' 1-74 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 0, R 1, C 3 N 1. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452846.

Wiscseq_DsLox4G07.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G13000.2-P355 and AT5G13010.1-P355 (at chr 5 pos 4121750 (C/4121750-4121830) on the TAIR10). The sequences' 1-81 bps mapped on the genome, 85-100 bps onto the T-DNA. Pools P 0, R 1, C 3 N 1. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO453146.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox4G07

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox4G07	WiscDsLox4G07	AT5G10070 AT5G13000 AT5G13010 At3g44050

EMBL accession:

CW792779