NASC Stock Detail Page
NASC ID: N850076
Name: WiscDsLox263B02
ABRC stock number: CS850076
Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line. Associated Polymorphisms: Wiscseq_DsLox263B02.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G22670.1 (at chr 5 pos 7538694 (C/7538694-7538783) on the TAIR10). The sequences' 1-90 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 1, R 0, C 3 N 4. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO452090. Wiscseq_DsLox263B02.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G05795 and AT5G05800.1-UTR3 (chr 5 pos 1742766 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797127. Wiscseq_DsLox263B02.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G47430.1 (chr 5 pos 19236846 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797126.
Donation Date: 2006-09-04
Donated by: | University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman |
Donor Number: WiscDsLox263B02
Part of : Wisconsin DsLox T-DNA lines
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-2
Transformation method: Agrobacterium
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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