NASC

NASC ID: N850933

Name: WiscDsLox303H06

ABRC stock number: CS850933

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox303H06 .

Wiscseq_DsLox303H06.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G47870.1 (chr 5 pos 19385877 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG798438.

Wiscseq_DsLox303H06.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G71450.1 (chr 1 pos 26926981 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG798435.

Wiscseq_DsLox303H06.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G50780.1-P355 and AT3G50790.1-UTR3 (chr 3 pos 18878090 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG798436.

Wiscseq_DsLox303H06.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G01400.1 (chr 5 pos 168202 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG798437.

Donation Date: 2006-09-04

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox303H06

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox303H06	WiscDsLox303H06	AT1G71450 AT3G50780 AT3G50790 AT5G01400 At5g47870

EMBL accession:

CW793767