NASC

NASC ID: N852309

Name: WiscDsLox351B09

ABRC stock number: CS852309

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox351B09 .

Wiscseq_DsLox351B09.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G53345.1-UTR3 and AT1G53350.1 (chr 1 pos 19903771 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG800330.

Wiscseq_DsLox351B09.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G53380.1-P355 and AT1G53390.1-P355 (at chr 1 pos 19917527 (C/19917527-19917597) on the TAIR10). The sequences' 15-85 bps mapped on the genome, 83-100 bps onto the T-DNA. Pools P 3, R 1, C 4 N 4. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO453036.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox351B09

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox351B09	WiscDsLox351B09	AT1G53345 AT1G53380 AT1G53390 At1g53350

EMBL accession:

CW793892