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NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N856036

Name: WiscDsLox442C5

ABRC stock number: CS856036

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Donation Date: 2004-12-01

Donated by:  University of Wisconsin, Madison Patrick Krysan
University of Wisconsin, Madison Richard Amasino
University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox442C5

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status Price (£)
Available to order at NASC £11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium