NASC

NASC ID: N856924

Name: WiscDsLox461-464K17

ABRC stock number: CS856924

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox461-464K17 .

Wiscseq_DsLox461-464K17.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G14226.1 and AT4G14230.1-UTR3 (chr 4 pos 8199769 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG806653.

Wiscseq_DsLox461-464K17.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G47740.1 (chr 1 pos 17566582 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG806651.

Wiscseq_DsLox461-464K17.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G62460 and AT1G62480.1-P355 (at chr 1 pos 23124289 (C/23124289-23124381) on the TAIR10). The sequences' 1-92 bps mapped on the genome, 84-100 bps onto the T-DNA. Pools P 7, R 4, C 2 N 4. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452814.

Wiscseq_DsLox461-464K17.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G73210.1 (chr 1 pos 27530253 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG806652.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox461-464K17

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox461-464K17	WiscDsLox461	AT1G47740 AT1G62460 AT1G62480 AT1G73210 AT4G14230 At4g14226