NASC

NASC ID: N856979

Name: WiscDsLox461-464M4

ABRC stock number: CS856979

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox461-464M4 .

Wiscseq_DsLox461-464M4.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT3G60966.1 (at chr 3 pos 22553438 (C/22553438-22553484) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 79-100 bps onto the T-DNA. Pools P 7, R 4, C 7 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO453716.

Wiscseq_DsLox461-464M4.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G63087.1 (chr 5 pos 25304818 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG806705.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox461-464M4

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox461-464M4	WiscDsLox461	AT3G60966 AT5G63087 At2g39420

EMBL accession:

CW798059