NASC

NASC ID: N858328

Name: WiscDsLox489-492I18

ABRC stock number: CS858328

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox489-492I18 .

Wiscseq_DsLox489-492I18.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G47060.1 (chr 2 pos 19333196 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG808503.

Wiscseq_DsLox489-492I18.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G28780.1-P355 and AT2G28790.1-UTR3 (chr 2 pos 12351839 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG808501.

Wiscseq_DsLox489-492I18.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G22620.2-P355 and AT2G22630.1 (chr 2 pos 9616185 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG808502.

Wiscseq_DsLox489-492I18.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G62130.1 (at chr 3 pos 23004733 (C/23004733-23004814) on the TAIR10). The sequences' 1-82 bps mapped on the genome, 83-100 bps onto the T-DNA. Pools P 8, R 7, C 9 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452250.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox489-492I18

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox489-492I18	WiscDsLox489	AT2G22620 AT2G22630 AT2G28780 AT2G28790 AT3G62130 At2g47060

EMBL accession:

CW802934